TY - JOUR DO - 10.31373/ejtcm/139693 TI - Multiplexed Immunobead-Based Cytokine Profiling in patients with ovarian cancer T2 - European Journal of Translational and Clinical Medicine AU - Wyciszkiewicz, Aleksandra AU - Lach, Michał AU - Kalinowska, Alicja AU - Michalak, Sławomir AU - Nowakowski, Błażej PY - 2021 DA - 2021/12/02 PB - Medical University of Gdańsk SP - 31 EP - 43 VL - 4 IS - 2 KW - ovarian cancer, cytokines, metalloproteinases, inflammation markers, endometriosis LA - en AB - Due to late diagnosis, ovarian cancer is the most deadly gynecologic malignancy. Inflammation is one of the risk factors of ovarian cancer and the inflammatory response implicates all stages of tumorigenesis. The purpose of this study was to analyze the concentration of molecules, which can take part in malignant processes. We analyzed patients with ovarian cancer, with endometriosis and healthy controls. Thirty-seven analytes were measured in serum using BioPlex Pro Human Inflammation Panel. We were able to detect 28 of the proteins among the studied groups. We found a significant increase in 22 of the tested molecules (BAFF, Chitinase3-like 1, IFN-alpha2, IFN-beta, IFN-gamma, IFN-lambda2, IFN-lambda1, gp130, IL-2, IL-12 (p40), IL-11, IL-32, IL-35, MMP3, Osteocalcin, Pantraxin-3, sCD163, TNFRSF8, sIL-6Ralpha, STNF-R1, STNF-R2, and TSLP) in the ovarian cancer group in comparison to the healthy controls. Two of them (IL-20, MMP1) did not show significant differences between groups. Moreover, we identified decreased concentrations of APRIL and osteopontin in ovarian cancer vs. healthy controls. While this study is a preliminary report, we hope this will encourage a further use of multiplex analysis in ovarian cancer biomarker research. LK - https://ejtcm.gumed.edu.pl/articles/139693 UR - https://doi.org/10.31373/ejtcm/139693 ER -