Inflammatory response to a marathon run in amateur athletes

Introduction

Statistics reveal a trend of increasing participation in mass endurance sports events, with the runners older and slower than ever before [1], which means that the number of amateur runners has increased. Amateur runners constitute a heterogeneous group in terms of fitness level, training regimen, medical history and cardiovascular risk factors. Moreover, the definition of an “amateur athlete” is not precise. The American Heart Association distinguishes elite, competitive and recreational athletes. The first and the second groups train with high intensity in organized teams with an emphasis on competition and performance, whereas the latter engage in sports activity for pleasure and in their spare time.

Regular exercise reduces the cardiovascular risk and all-cause mortality, with a 20-30% reduction in cardiovascular adverse events compared with patients who have sedentary lifestyle. The current European Society of Cardiology guidelines recommend a minimum of 150 min of exercise of moderate-intensity over 5 days or 75min of vigorous exercise over 3 days per week for a healthy adult [2].

Although the benefits of regular moderate intensity exercise remain indisputable, there is a concern that the long duration high-intensity endurance sports may elicit negative effects on the heart by triggering the structural, functional and electrical remodeling, hence increasing the risk of arrhythmias. One of the postulated mechanisms is the inflammatory response following an intensive endurance exercise [3]. Sorokin et al. in their review showed that endurance-trained athletes are at increased risk of developing atrial fibrillation with the possible mechanisms being increased parasympathetic tone, increased atrial size and increased inflammatory reaction [4]. There are also reports that high-intensity leisure-time physical activity by stimulating the inflammatory reaction may contribute to the development of atherosclerosis in the long run [5].

For over 50 years, the utility of various biomarkers in the diagnosis of cardiovascular diseases was analyzed [6], yet their implications still remain not fully understood. Many novel biomarkers were recently discovered including inflammatory biomarkers such as pentraxin-3 (PTX-3) and neopterin. However, there is a lack of data about amateur athletes as to whether such sport activities are associated with the activation of an inflammatory reaction. The aim of this study was to investigate the effect of running a marathon on the inflammatory response in the group of male amateur runners.

Material and Methods

The study was carried out on a group of 40 male amateur marathoners, who competed in and finished the 2nd PZU Marathon in Gdańsk, Poland. The participation in the study was voluntary. Enrolment into the study was completed via invitations sent to sports clubs. Each participant signed a written consent form prior to enrolment. The study protocol was approved by Independent Bioethics Committee for Scientific Research at Medical University of Gdańsk (No. NKBBN 104/2016). Information about health and training conditions was gathered via structured interviews. Exclusion criteria were: history of past or chronic illness/es. After the finishing the marathon run, we asked the participants to suspend high-intensity training as well as participation in any upcoming competitions. The characteristics of a study group were described previously [7].

We divided the study into three stages. Blood samples from the cubital vein were collected at each stage. Stage 1 was carried out 2 weeks before the run, Stage 2 directly after finishing the run on the finish line and Stage 3 took place 2 weeks after the marathon. Fasting blood samples at Stage 1 and Stage 3 were collected at the cardiology department. Serum was prepared immediately after collection by centrifugation at 2000 rpm at room temperature for 12 minutes and then stored in –80˚C for the further analysis [7].

Samples from each stage were analyzed in terms of the amount of leukocytes, neutrocytes, lymphocytes, monocytes, eosinophils, basophils, immature granulocytes and the concentration of fibrinogen and creatine kinase. Biochemical parameters were analyzed using Architec c8000 (Abbott). Endothelin-1 (ET-1) concentration was measured using a solid phase sandwich Quantikine ELISA (R&D Systems) with sensitivity of 0.207 pg/ml and detection range from 0.39 to 25 pg/ ml. PTX-3 concentration was measured using a solid phase sandwich ELISA Human Pentraxin 3/TSG-14 DuoSet (R&D Systems) with detection range from 218 to 14000 pg/ml. Neopterin concentration was measured using a solid phase competition ELISA (Demeditec Diagnostics) with sensitivity of 0.7 nmol/l and detection range from 1.35 to 111 nmol/l. Interleukin 6 (IL-6) concentration was measured using a solid phase sandwich Quantikine ELISA (R&D Systems) with a sensitivity of 0.7 pg/ml and detection range from 3.1 to 300 pg/ml.

Continuous variables were expressed as means ± standard deviation (SD). Before the statistical analyses, Shapiro-Wilk test was used to test the normal distribution of variables. Analysis of variance (ANOVA) for repeated measures was used to test statistical differences between groups of variables. Post-hoc analysis was performed with a Tukey's test. For the variables analyzed at two stages only, the t-test for dependent variables was used. The data was analyzed using Statistica 12 software (StatSoft). A p value < 0.05 was considered statistically significant [7].

Results

Study group

The characteristics of the studied group are presented in Table 1 [7].

Table 1. Characteristics of the studied group

Biochemical analysis

The results of the analysis of white blood cells counts fibrinogen and creatine kinase concentrations are presented in Table 2.

Table 2. Biochemical analysis of blood samples collected 2 weeks before the marathon (S1), at the finish line (S2) and 2 weeks after the marathon (S3)

Mean leukocyte count at Stage 1 was 5.8 G/l. At Stage 2 it was 16.5 G/l and it differed significantly from the results at Stage 1 and Stage 3. There was no significant difference between leukocyte count at Stage 1 and Stage 3. The same trend was observed for neutrocytes, lymphocytes, monocytes, eosinophils, basophils and immature granulocytes, as well as for fibrinogen and creatine kinase concentrations. The concentration of creatine kinase at Stage 2 was significantly higher compared to Stage 1 and Stage 3.

Biomarkers

Table 3 shows the concentrations of the analyzed biomarkers.

Table 3. Concentrations of cardiac injury and overload biomarkers: 2 weeks before the marathon (S1), at the finish line (S2) and 2 weeks after the marathon (S3)

Interleukin 6 (IL-6), not detectable (ND)

The mean concentration of ET-1 was the highest at Stage 2 (3.2 ± 0.9 pg/ml) and it differed significantly from both Stage 1 and Stage 3. Neopterin showed the same trend. PTX-3 concentrations differed significantly between all the stages, with the highest concentration at Stage 2 and the lowest at Stage 3. The concentration of IL-6 was significantly higher at Stage 2 compared to Stage 1 and exceeded the norm many times (Norm: lower or equal to 1.8 pg/ml). At S3 the mean concentrations of IL-6 were undetectable.

Discussion

In our study, we found that running a marathon increased the inflammatory response in amateur runners. This was probably due to skeletal muscle damage, as inflammatory biomarkers were normalizing 2 weeks after the run. To our knowledge, this is the first study to demonstrate the impact of a bout of intense exercise on the inflammatory response in male amateur marathoners, assessed on the basis of changes in PTX-3 and neopterin concentrations.

In our study group, increased concentrations of creatine kinase at Stage 2 suggest exercise-induced muscle damage. This was accompanied by a significant increase in concentrations of all the studied biomarkers, compared with Stage 1 and Stage 3. At Stage 2 significant leukocytosis with an increase in all leukocyte-fractions was also observed. Kosowski et al. investigated cardiovascular stress biomarkers in middle-aged non-athlete marathon runners. Blood samples were collected (before, just after and 7 days after the marathon) and analyzed for endothelin-1, troponin I and N-terminal pro B-type natriuretic peptide concentrations. The authors concluded that the marathon was associated with a significant increase in cardiovascular stress biomarkers but the profile of these changes did not suggest irreversible myocardial damage [8].

It has been suggested that completing a marathon has similar physiological sequelae to the acute-phase response: neutrophil leucocytosis, increased creatine kinase activity, a rise in C-reactive protein and fibrinogen levels and an increase in plasma cortisol concentration [9]. On the other hand, significant increases of the creatine kinase concentration and elevation of inflammatory markers have been observed after prolonged cardiopulmonary resuscitation [10] or direct current cardioversion [11].

Endothelin-1, which is released by leukocytes, macrophages and fibroblasts [12], is not only a potent vasoconstrictor of the smooth muscle cells but it also has a pro-inflammatory effect [13, 14]. Its expression is increased in response to cytokines, reactive oxygen species, angiotensin II and thrombin [15]. ET-1 has been shown to stimulate monocytes to produce interleukin-8 (IL-8) and monocyte chemoattractant protein-1 (MCP-1)[16], and also acts as a mast cell activator resulting in the release of inflammatory cytokines such as tumor necrosis factor alpha (TNF-alpha) and IL-6 [17]. A study of mice showed that intensive endurance exercise increases the occurrence of atrial fibrillation in a mechanism of inflammation and atrial fibrosis with the involvement of a soluble TNF-alpha signaling pathway [18]. More than a 33-fold increase in the mean concentration of IL-6 at S2 compared to the baseline value is consistent with the observations of Pinho et al. [19] (a group of Ironman race participants) and Schobersberger et al. [15] (participants of an ultramarathon). Intensive physical exercise causes an increase in oxygen consumption and induces oxidative stress due to free radical production, which in turn stimulates cytokine production from various cell types and upregulates the inflammatory cascade [20-21].

Neopterin is a biomarker of the cellular immune response released by activated macrophages and dendritic cells upon activation with gamma-interferon and acts as a modulator and mediator in inflammatory and infectious processes [22]. A significant increase in the post-run concentration of neopterin is consistent with the observations published by Schobersberger et al. [23] and Sprenger et al. [24] who examined well-trained runners after running 20 km in 2 hours. The pentraxins superfamily comprises of short and long pentraxins. PTX-3 is a member of long pentraxin group and is believed to play a regulatory role in innate immunity, sterile and non-sterile inflammation, tissue repair, and cancer [25]. PTX-3 is an acute phase protein, produced locally by monocytes, endothelial cells and fibroblasts in response to pro-inflammatory signals like interleukin 1 beta or TNF alpha. The major source of PTX-3 are vascular endothelial cells [26]. Increased plasma levels of PTX-3 were found in patients with acute myocardial infarction, heart failure, atherosclerosis and after cardiac arrest. Salio et al. indicated that PTX-3 plays a protective role against myocardial ischemia in their study on a mouse model of myocardial infarction. PTX3-deficient mice showed exacerbated cardiac damage with greater no-reflow area, increased neutrophil aggregation, decreased number of capillaries and increased number of apoptotic cardiomyocytes [27].

The influence of intense endurance exercise on plasma concentrations of PTX-3 in humans has not yet been extensively studied. Miyaki et al. measured plasma PTX-3 concentrations in young male endurance runners and sedentary controls and found higher concentrations in the first group as a result of a postulated training-induced cardioprotection [28]. In contrast, Suzuki et al. showed a hypertrophic response and left ventricular systolic dysfunction as a consequence of increased afterload in a mouse model of transverse aortic constriction. Transverse aortic constriction (TAC) is one of the most common surgical models of pressure overload-induced cardiac hypertrophy and heart failure. In the TAC model, a permanent constriction is placed around the transverse aorta, limiting left ventricular outflow and thereby creating pressure overload in the left ventricle. Echocardiography indicated that PTX-3 overexpression promoted tissue remodelling, left ventricular systolic dysfunction and myocardial fibrosis, while these responses were suppressed in PTX3-deficient mice [29].

Two weeks after the marathon, white blood cell counts, creatine kinase and fibrinogen levels, as well as ET-1 and neopterin concentrations returned to baseline, and PTX-3 level fell below the Stage 1 value. The concentration of IL-6 at S3 was undetectable. We explain this by the lack of intensive training or participation in any sporting events between S2 and S3 compared to the pre-marathon preparation period according to the study protocol. This trend suggests that a marathon run does not cause a prolonged inflammatory response.

On the other hand, numerous studies have reported that health benefits from extreme forms of physical activity such as ultra-endurance sports, are attenuated in an inverted J-curve dose-response model, with increased risk of adverse ventricular remodeling, fibrosis and arrhythmias. La Gerche emphasizes the phenomenon of cardiac overtraining in the potential mechanism of arrhythmogenesis in endurance athletes, in which a chronic adverse cardiac remodeling depicts an imbalance between the exercise-induced injury and an insufficient period of regeneration [30-31].

Kwaśniewska et al. observed the population of physically active men for over 25 years and reported that the most favourable effect against atherosclerosis was associated with energy expenditure between 2050 and 3840 kcal/week. Regular and very high physical activity was accompanied by the deterioration of the examined indicators of atherosclerosis (increased calcification of the coronary arteries and intima-media thickness). The authors postulated that intense physical activity in free time may be associated with the intensification of low-grade inflammation and thus has a pro-atherosclerotic effect [5].

Currently, there is little data available regarding the long-term effects of intense endurance activity on the inflammatory response. However, a prospective, long-term study of at least 130 marathon runners is currently underway by Schoenfeld et al. [32] and may provide important information on this topic. The aim of this study is to assess the physiological response of the cardiovascular system and potential abnormalities after 10 years of long-term vigorous endurance exercise.

The main limitation of our study is the lack of a long-time observation. The biomarker kinetics were not monitored in the time interval between S2 and S3. Secondly, the study was conducted on a relatively small group of male participants only. Finally, we did not include echocardiographic imaging, however this was not the purpose of this part of the study.

Conclusions

Our study appears to be the first to investigate the changes in PTX-3 and neopterin concentrations in amateur athletes. We demonstrated that male amateur marathon runners follow similar trends in inflammation biomarker kinetics as in Ironmen, ultramarathoners and elite athletes. Intensive endurance exercise causes an acute transient rise in the concentrations of inflammatory biomarkers in amateur marathon runners together with leukocytosis and increased creatine kinase. In the short-term follow-up, the concentrations of all studied parameters normalized, suggesting that the inflammatory cascade is mainly induced by exercise-induced muscular damage. Further research is needed to investigate the long-term effects of recurrent exercise-induced inflammation on the cardiovascular system.

Acknowledgements: None declared.

Statement of competing interests: None declared.